A multi-factor experimental design for evaluating random-access analyzers.

J S Krouwer; W N Stewart; B Schlain

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PubMed · 3416441

A multi-factor experimental design for evaluating random-access analyzers.

J S Krouwer·W N Stewart·B Schlain

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Abstract

A multi-factor experimental design for evaluating random-access analyzers has been developed and tested for the Ciba Corning "550 Express" random-access analyzer. The 12-sample design estimates imprecision, slope, nonlinearity, linear drift, and reagent carryover to the next assay. The design was constructed so that estimates of the factors' effects are almost entirely uninfluenced by each other. Use of the design is illustrated by an example in which reagent-to-assay carryover was pinpointed as an apparent cause of high imprecision. This led to a modification of the analyzer such that carryover was insignificant. The appendix contains a 27-sample design that provides additional estimates. Software to perform such calculations is available on request.

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J S Krouwer, W N Stewart, B Schlain. 1988. A multi-factor experimental design for evaluating random-access analyzers.. https://pubmed.ncbi.nlm.nih.gov/3416441/

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