PubMed · 3370591
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1988-06-15. Migraine.. https://pubmed.ncbi.nlm.nih.gov/3370591/
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Protoplasts isolated from tomato, wild tomato, barley and chrysanthemum were electrotransfected with tobacco mosaic virus (TMV) RNA under almost the same optimum electric conditions: five square DC pulses of 50 microseconds duration at 500 to 800 V/cm, with the protoplasts suspended at 2 x 10(5)/ml in 0.5 M-mannitol containing 100 microM-MgCl2 and 10 to 20 micrograms/ml TMV RNA. ELISAs of these transfected protoplasts showed that the yields and the growth curves of the virus were quite similar, indicating a lack of host specificity in the initially infected cells of these plants.
Pollinosis, first of all mugwort pollinosis, due to pollens of wind pollinated Asteraceae is not seldom. On the other hand allergic reactions to pollens of insect pollinated members of this plant family are a rarity. 8 cases of pollinosis to Chrysanthemums in a floriculture farm are offered, caused by the work specific conditions of exposure in artificial pollination. The analysis of allergens and RAST inhibition tests showed us a close relationship of allergens of Chrysanthemum pollens and pollens of mugwort. It was possible to continue cultivating work in all cases because of several protective measures and hyposensitization.
We developed a dot-blot hydridization assay for the detection of Chrysanthemum stunt viroid (CSV) in Chrysanthemum plant samples. The probe, a recombinant plasmid containing a full-length monomeric cDNA copy of CSV, is labelled with (32P) by nick-translation. The influence of the hybridization conditions, of the sample denaturation technique and of the plant sap components on the final sensitivity has been studied. The optimized system, involving a formaldehyde denaturation step, allows the detection of as little as 5 pg of purified viroid. Under these conditions, 100 pg of pure viroid diluted in plant sap, or infected plant extract diluted 1:25 in healthy extract can be detected, showing the potential of this method for indexing of Chrysanthemum for CSV infection.