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Intermediates in plasmid pT181 DNA replication.

S Majumder·R P Novick
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Abstract

Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali denaturation. A model for pT181 replication is proposed to accommodate this apparent discrepancy.

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Anti-Bacterial AgentsBacterial ProteinsDNA ReplicationDNA, BacterialPlasmidsRepliconStaphylococcus aureusJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S Majumder, R P Novick. 1988-04-11. Intermediates in plasmid pT181 DNA replication.. https://doi.org/10.1093/nar%2F16.7.2897

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