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PubMed · 329477

A hydrostatic device for tissue dehydration.

D Goldfarb·K Miyai·J Hegenauer
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Abstract

A simple device for mixing water and organic solvents can be easily constructed to dehydrate tissue for electron microscopy. The device utilizes hydrostatic leveling between a reservoir and a mixing chamber to produce a continuously increasing concentration of dehydrating solvent. The rate of dehydration may be regulated by geometry and/or outflow rate. A prototype model is described which compactly incorporates the solvent reservoirs and a tissue tray that can accommodate ten tissue specimens in stainless steel mesh baskets. The device can be fabricated cheaply and replaces manual dehydration.

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DesiccationHistological TechniquesHydrostatic PressureMethodsMicroscopy, ElectronSolventsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D Goldfarb, K Miyai, J Hegenauer. 1977. A hydrostatic device for tissue dehydration.. https://doi.org/10.3109/10520297709116770

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