PubMed · 3234612
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N Gordon. 1988. Intractable epilepsy.. https://doi.org/10.1111/j.1469-8749.1988.tb14649.x
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Tiagabine x HCl is being developed as an anti-convulsant/anti-epileptic agent for seizure disorders. The pharmacological activity of the R-(-)-enantiomer is higher than that of the S-(+)-enantiomer. Therefore, the drug is synthesized in the pure R-(-)-enantiomeric form. The enantiomers of tiagabine x HCl were separated on a modified cellulose stationary phase (Chiralcel-OD) with a mobile phase of hexane-isopropanol-ethanol (80:14:06, v/v/v). Approximately 5 ml of trifluoroacetic acid was added for each liter of the mobile phase mixture. The method is capable of separating the two enantiomers with a selectivity factor of 1.55 and a resolution factor of 3.4. The samples of tiagabine x HCl were monitored by a UV detector at 260 nm. The method was validated by conducting standard addition and recovery of the S-(+)-enantiomer in tiagabine x HCl. The R.S.D. of the method is 3.2%. The limit of quantification (LOQ) of the S-(+)-enantiomer present in tiagabine x HCl is about 0.03%.
A new method for the preparation of restricted access media (RAM) was developed. A low-temperature plasma treatment removed octadecyl groups on the external surface of octadecylsilylated silica gel before those on the internal surface to produce silanol groups. The silanol groups produced were glycerylpropylsilylated to give RAM, DIOL-ODSs, consisting of internal octadecyl groups and external glycerylpropyl groups. DIOL-ODSs were inert to serum proteins and retained low-molecular-mass analytes adequately. Direct injection analysis of anticonvulsants in serum was accurately performed by column-switching high-performance liquid chromatography using a pre-column packed with DIOL-ODS.