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PubMed · 322892

Solid-phase enzyme immunoassay for hepatitis B surface antigen.

R Wei·G J Knight·D H Zimmerman·H E Bond
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Abstract

A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.

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False Positive ReactionsHepatitis B Surface AntigensHumansImmunoenzyme TechniquesMethodsRadioimmunoassayComparative StudyJournal Article

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R Wei, G J Knight, D H Zimmerman, H E Bond. 1977. Solid-phase enzyme immunoassay for hepatitis B surface antigen.. https://pubmed.ncbi.nlm.nih.gov/322892/

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