PubMed · 3195020
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M H Fussell. 1988-09-24. Probiotics.. https://doi.org/10.1136/vr.123.13.354
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A national program to reduce Salmonella in pork and pork products should include monitoring and intervention at farm level. To develop an adequate intervention strategy at farm level, risk factors for Salmonella infections in finishing pigs have to be determined. In this study, blood samples were collected randomly at two slaughterhouses from slaughter pigs. Samples were tested by the Dutch Salmonella ELISA, based on the O-antigens 1, 4, 5, 6, 7 and 12, using a cut-off of OD%=10. This ELISA has been calibrated against the Danish ELISA to give comparable results. Workers from herds from which at least forty blood samples had been collected, were asked to participate in a questionnaire. In total, 353 questionnaires were obtained and analysed. Significant risk factors associated with the proportion of seropositive samples were identified by multiple linear logistic regression. The feeding of a complete liquid feed containing fermented by-products and the omission of disinfection after pressure washing a compartment as part of an all-in/all-out procedure, were both associated with a lower Salmonella seroprevalence. A small to moderate herd size (<800 finishing pigs), a previous diagnosis of clinical Salmonella infection in the herd, the use of tylosin as an antimicrobial growth promoter in finishing feed, or herds which had more than 16% of the livers of their pigs condemned at the slaughterhouse as a result of white spots were associated with a higher Salmonella seroprevalence. Hypothetical intervention strategies based on these risk factors can be studied for their effect on the Salmonella seroprevalence and practical applicability in field studies.
We ran a controlled intervention trial to assess whether the risk of a broiler flock becoming infected with Campylobacter could be reduced by biosecurity measures. These were a standard method of cleansing and disinfecting the poultry house prior to stocking, and a standard hygiene protocol followed by all personnel who entered the study house during the flock's life. Thirty-nine flocks were allocated to intervention or control groups in a ratio of 1:2. Intervention flocks were asked to follow the specified biosecurity measures; all flocks were monitored weekly for Campylobacter infection. Analysis of infection at 42 days of age and over the life of the flock showed that the risk of thermophilic Campylobacter infection of broilers was reduced by over 50% in intervention flocks. Parts of the intervention identified as significant in the univariable analysis included twice weekly replenishment of boot dip disinfectant; potential independent risk factors identified included the location of ventilation fans and daily sanitisation of the water supply. The non-random allocation of 10 flocks to the control group may have introduced some study bias (the effect of which is discussed in the paper).
Colony lineages from three Mycoplasma bovis outbreaks representing different husbandry conditions in the United States were characterized with arbitrarily primed polymerase chain reaction (AP-PCR). Cases studied included a closed beef herd, a dairy calf ranch, and a feedlot. The DNA was obtained from colony lineages and used for AP-PCR with primers REP1R-I and REP2-I. Case A and C lineages were uniform by AP-PCR analysis. Lineages from case B showed heterogeneity with AP-PCR. Outbreaks A and C were therefore both infected by one source, while the ranch (case B) was infected by multiple calf shipments. The AP-PCR typing method provides genotypic epidemiological information to successfully characterize M. bovis from sequential sampling of outbreaks and different husbandry conditions.