Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 3104211

Buffer kinetics in biofiltration.

M Feriani·L Bragantini·R Dell'Aquila·S Chiaramonte·A Fabris·S Biasioli·C Ronco·A Brendolan·G La Greca
View original source ↗Export metadata ↓

Abstract

A condition of metabolic alkalosis has been generally observed in patients undergoing regular biofiltration (BF). The aim of this study was to assess buffer kinetics during a dialysis session in five patients regularly treated by BF for 10 months (dialyzer Biospal 3000S, Qb 301; Qd 545; UF 36.76 ml/min; dialysate CH3 COO 38 mEq/L; replacement fluid 1000 ml/h with HCO3 100 mEq/L solution). As usually happens using dialyzers with high surface area and permeability, large HCO3 losses (756 +/- 112 mEq) and CH3COO uptakes (677 +/- 152 mEq) were observed. Acetate plasma levels rose to 10.4 mEq/L, a value potentially dangerous to the cardiovascular system. The dialytic buffer gain (acetate uptake + HCO3 administered - HCO3 loss) was high (230 +/- 137 mEq/dialysis) and excessive for patients' needs. It is therefore very important to reduce either the acetate concentration in dialysate or the amount of reinfused bicarbonate. In view of the plasma acetate levels it is preferable to use a dialysis solution containing less acetate.

Explore related subjects

AcetatesAcid-Base EquilibriumAdultBicarbonatesBloodBuffersCarbon DioxideFemaleHumansKineticsMiddle AgedOxygenRenal DialysisUltrafiltrationJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

M Feriani, L Bragantini, R Dell'Aquila, S Chiaramonte, A Fabris, S Biasioli, C Ronco, A Brendolan, G La Greca. 1986. Buffer kinetics in biofiltration.. https://pubmed.ncbi.nlm.nih.gov/3104211/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1997

The effect of novel anti-epileptic drugs in rat experimental models of acute and chronic pain.

The novel anti-epileptic drugs lamotrigine, felbamate and gabapentin were compared in rat experimental models of acute (tail flick) and chronic pain: the chronic constriction injury and spinal nerve ligation models. Lamotrigine (10-100 mg/kg, s.c.), felbamate (150-600 mg/kg, i.p.) and gabapentin (30-300 mg/kg, i.p.) each reversed cold allodynia (chronic constriction injury model) with ED50 values of 28, 241 and 103 mg/kg, respectively, 1 h post-dose. However, only gabapentin reversed tactile allodynia (spinal nerve ligation model) with an ED50 of 34 mg/kg (i.p.). The established anti-epileptic drugs, carbamazepine (1-30 mg/kg, i.p.) and phenytoin (1-100 mg/kg, s.c.), were ineffective in both models. The anti-allodynic effect of the newer anti-epileptic drugs was observed at doses that were either ineffective or produced only a negligible effect on acute nociceptive function and/or locomotor activity. In conclusion, the data suggest that the newer anti-epileptic drugs appear to have the potential to be effective alternatives to either carbamazepine or phenytoin in the treatment of neuropathic pain. However, only gabapentin ameliorated both cold and touch hyperesthesias.

Acetates↗
PubMed1997

Capillary gas-liquid chromatography of acetate-methyl esters of bile acids.

Gas-liquid chromatographic separations of acetate-methyl esters of several common bile acids with and without a hydroxyl group at C-6 are compared with those of the corresponding trimethylsilyl ether-methyl esters on a CP-Sil-5 CB capillary column. Unlike the trimethylsilyl ether derivatives, the retention indices of the corresponding acetates were greatly influenced by the number of hydroxyl groups in the ring system. Epimeric hydroxyl groups at carbons 6, 7 as well as 12 increased retention index of the acetate-methyl esters of the bile acids, the effect of the 7 beta-hydroxyl group being most prominent. The 6 beta-acetoxyl group increased the retention index more than the 6 alpha-acetoxy group and contrary to the trimethylsilyl ether derivatives, a 6 beta, 7 beta-diacetoxy group showed larger increase in the retention index than the corresponding 6 alpha, 7 beta-diacetoxy group. The acetate derivatives of bile acid-methyl esters show larger retention times and reduced sensitivity than the corresponding trimethylsilyl ether derivatives. However, gas chromatography of bile acid acetate-methyl esters can be very useful for the characterization of bile acids and for bile acid analysis in the rat where muricholic acids and hyodeoxycholic acid are in abundance, since these bile acids are difficult to resolve from each other and from other common bile acids as the trimethylsilyl ether derivatives.

Acetates↗
PubMed1997

Growth of Escherichia coli in acetate as a sole carbon source is inhibited by ankyrin-like repeats present in the 2',5'-linked oligoadenylate-dependent human RNase L enzyme.

Expression of low levels of the 2',5'-linked oligoadenylate-dependent human RNase L, an enzyme induced by interferons, is highly toxic in Escherichia coli. This protein contains an ankyrin domain responsible for RNase L toxicity. The only known ORF in E. coli containing ankyrin repeats is yjaC in the acetate metabolic cluster. We have investigated if expression of mutant forms of RNase L interfere with metabolism of acetate in E. coli. Our findings demonstrate that E. coli expressing RNase L ankyrin repeats is unable to grow in medium containing acetate as the sole carbon source, while it can grow when expressing other domains of the protein. This defect correlates with a severe decrease in the levels of induction of enzymes in the glyoxylate bypass.

Acetates↗