PubMed · 3055848
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K Theiler. 1988. Vertebral malformations.. https://doi.org/10.1007/978-3-642-73775-6
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The phylogenetic relationships among the three orders of modern amphibians (Caudata, Gymnophiona, and Anura) have been estimated based on both morphological and molecular evidence. Most morphological and paleontological studies of living and fossil amphibians support the hypothesis that salamanders and frogs are sister lineages (the Batrachia hypothesis) and that caecilians are more distantly related. Previous interpretations of molecular data based on nuclear and mitochondrial rRNA sequences suggested that salamanders and caecilians are sister groups to the exclusion of frogs. In an attempt to resolve this apparent conflict, the complete mitochondrial genomes of a salamander (Mertensiella luschani) and a caecilian (Typhlonectes natans) were determined (16,656 and 17,005 bp, respectively) and compared with previously published sequences from a frog (Xenopus laevis) and several other groups of vertebrates. Phylogenetic analyses of the mitochondrial data supported with high bootstrap values the monophyly of living amphibians with respect to other living groups of tetrapods, and a sister group relationship of salamanders and frogs. The lack of phylogenetically informative sites in the previous rRNA data sets (because of its shorter size and higher among-site rate variation) likely explains the discrepancy between our results and those based on previous molecular data. Strong support of the Batrachia hypothesis from both molecule- and morphology-based studies provides a robust phylogenetic framework that will be helpful to comparative studies among the three living orders of amphibians and will permit better understanding of the considerably divergent vertebral, brain, and digit developmental patterns found in frogs and salamanders.
The present study is the first of a series on descending supraspinal pathways in amphibians in which hodologic and developmental aspects are studied. Representative species of anurans (the green frog, Rana perezi, and the clawed toad, Xenopus laevis), urodeles (the Iberian ribbed newt, Pleurodeles waltl), and gymnophionans (the Mexican caecilian, Dermophis mexicanus) have been used. By means of retrograde tracing with dextran amines, previous data in anurans were largely confirmed and extended, but the studies in P. waltl and D. mexicanus present the first detailed data on descending pathways to the spinal cord in urodeles and gymnophionans. In all three orders, extensive brainstem-spinal pathways were present with only minor representation of spinal projections originating in forebrain regions. In the rhombencephalon, spinal projections arise from the reticular formation, several parts of the octavolateral area, the locus coeruleus, the laterodorsal tegmental nucleus, the raphe nucleus, sensory nuclei (trigeminal sensory nuclei and the dorsal column nucleus), and the nucleus of the solitary tract. In all species studied, the cerebellar nucleus and scattered cerebellar cells innervate the spinal cord, predominantly contralaterally. Mesencephalic projections include modest tectospinal projections, torospinal projections, and extensive tegmentospinal projections. The tegmentospinal projections include projections from the nucleus of Edinger-Westphal, the red nucleus, and from anterodorsal, anteroventral, and posteroventral tegmental nuclei. In the forebrain, diencephalospinal projections originate in the ventral thalamus, posterior tubercle, the pretectal region, and the interstitial nucleus of the fasciculus longitudinalis medialis. The most rostrally located cells of origin of descending spinal pathways were found in the suprachiasmatic nucleus, the preoptic area and a subpallial region in the caudal telencephalic hemisphere, probably belonging to the amygdaloid complex. Our data are discussed in an evolutionary perspective.
Immunohistochemical studies with antibodies against tyrosine hydroxylase, dopamine, and noradrenaline have revealed that the spinal cord of anuran, urodele, and gymnophionan (apodan) amphibians is abundantly innervated by catecholaminergic (CA) fibers and terminals. Because intraspinal cells occur in all three orders of amphibians CA, it is unclear to what extent the CA innervation of the spinal cord is of supraspinal origin. In a previous study, we showed that many cell groups throughout the forebrain and brainstem project to the spinal cord of two anurans (the green frog, Rana perezi, and the clawed toad, Xenopus laevis), a urodele (the Iberian ribbed newt, Pleurodeles waltl), and a gymnophionan (the Mexican caecilian, Dermophis mexicanus). To determine the exact site of origin of the supraspinal CA innervation of the amphibian spinal cord, retrograde tracing techniques were combined with immunohistochemistry for tyrosine hydroxylase in the same sections. The double-labeling experiments demonstrated that four brain centers provide CA innervation to the amphibian spinal cord: 1.) the ventrolateral component of the posterior tubercle in the mammillary region, 2.) the periventricular nucleus of the zona incerta in the ventral thalamus, 3.) the locus coeruleus, and 4.) the nucleus of the solitary tract. This pattern holds for all three orders of amphibians, except for the CA projection from the nucleus of the solitary tract in gymnophionans. There are differences in the strength of the projections (based on the number of double-labeled cells), but in general, spinal functions in amphibians are controlled by CA innervation from brain centers that can easily be compared with their counterparts in amniotes. The organization of the CA input to the spinal cord of amphibians is largely similar to that described for mammals. Nevertheless, by using a segmental approach of the CNS, a remarkable difference was observed with respect to the diencephalic CA projections.