PubMed · 2969987
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E Ishikawa. 1988. [Microchemistry by EIA].. https://pubmed.ncbi.nlm.nih.gov/2969987/
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Immunohistochemistry of the human hair follicle is of increasing interest in hair research. The data on antigen distribution in the different epithelial and mesenchymal structures of this unique skin appendage are superfluous now. In the present chapter, I will concentrate on selected aspects related to hair follicle differentiation, epithelial-mesenchymal interactions, proliferation and metabolic activity. Hair diseases are common. Not unusually, hair growth and structure reflect systemic disturbances. Basic knowledge of hair anatomy and histochemistry is required for their rational evaluation. Immunohistochemistry is a valuable tool for microanatomy of the hair apparatus. It offers a link between the biochemical data and structural components of hair follicles, which may help to better understand physiology of hair growth and hair diseases.
Thrombomodulin (TM), an endothelial receptor for thrombin, endowed with a powerful anticoagulant activity, plays an important role in the antithrombogenicity of the vascular endothelium. Its presence within the human renal glomerulus is already known but was thought to be only endothelial. We looked for TM expression in human mesangial cells (MC), both in situ, in freshly prepared glomeruli, and in primary culture. Both fresh and cultured MC were strongly reactive for TM by immunocytochemical methods. Total TM antigen measured on MC lysates and surface TM activity on MC were 0.292 +/- 0.075 ng/mg of cellular proteins and 1.20 +/- 0.02 pmole of activated protein C/min/mg of cellular proteins, respectively. As shown by the presence of numerous transcripts detected by in situ hybridization, TM was shown to be synthesized by MC in vivo and in culture. The synthesis of active TM by both endothelial and mesangial cells within the renal glomerulus stresses the importance of its role in maintaining renal hemostatic equilibrium, and sheds some light on the conflicting reports of TM over- and underexpression in glomerulopathies to open a new field for investigation.
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