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PubMed · 2958273

Decrease in IGF-I binding sites on human promyelocytic leukemia cell line (HL-60) with differentiation.

I Sukegawa·N Hizuka·K Takano·K Asakawa·K Shizume
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Abstract

Specific insulin-like growth factor I (IGF-I) receptors on human promyelocytic leukemia cell line (HL-60) were identified and characterized. [125I]IGF-I specifically bound to the cells, and [125I]IGF-I binding to the cells was displaced by unlabeled IGF-I in a dose dependent manner. [125I]IGF-I binding to the cells were displaced by multiplication stimulating activity (MSA) and porcine insulin, with potencies that were 10 and 100 times less than that of IGF-I, respectively. By an affinity labeling technique, IGF type I receptors were found to be present on the HL-60 cells. After the cells were differentiated to the macrophage-like cells by 12-o-tetra-decanoyl-phorbol-13-acetate (TPA) and 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3), [125I]IGF-I binding to the cells decreased significantly. By Scatchard analysis, it was found to be due to a decrease in the number of IGF-I receptors. Thus, the differentiation of HL-60 cells to the macrophage-like cells was accompanied by a decrease in IGF-I receptors.

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Affinity LabelsCell DifferentiationCell LineHumansInsulin-Like Growth Factor ILeukemia, Myeloid, AcuteMacrophagesReceptor, InsulinReceptors, SomatomedinSomatomedinsJournal ArticleResearch Support, Non-U.S. Gov't

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I Sukegawa, N Hizuka, K Takano, K Asakawa, K Shizume. 1987. Decrease in IGF-I binding sites on human promyelocytic leukemia cell line (HL-60) with differentiation.. https://doi.org/10.1507/endocrj1954.34.365

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