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PubMed · 2850037

[A decrease in the stimulated level of free intracellular calcium in thrombocytes during refractoriness].

E Ia Pozin·E G Popov·I Iu Gavrilov·Z A Gabbasov·S N Krotov
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Abstract

The concentration of free intracellular calcium (Ca.f) was determined in ADP-stimulated platelets. Free calcium was measured by the multichannel method using fluorescent probe indo 1. In the presence of extracellular calcium, ADP induced a rapid reversible increase in Ca.f followed by reversible platelet aggregation. The second addition of ADP also induced increase in Ca.f and platelet aggregation, which were of lesser magnitude (platelet refractoriness). Without extracellular calcium, ADP also produced an increase in Ca.f. In this case the second addition of ADP also induced considerably less increase in Ca.f.

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Adenosine DiphosphateAnimalsBlood PlateletsCalciumCyclic AMPCytoplasmFluorescent DyesPlatelet AggregationRabbitsStimulation, ChemicalComparative StudyEnglish AbstractJournal Article

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E Ia Pozin, E G Popov, I Iu Gavrilov, Z A Gabbasov, S N Krotov. 1988. [A decrease in the stimulated level of free intracellular calcium in thrombocytes during refractoriness].. https://pubmed.ncbi.nlm.nih.gov/2850037/

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