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PubMed · 2848772

Thiourea and dimethylthiourea decrease human neutrophil bactericidal function in vitro.

J H Jackson·E M Berger·J E Repine
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Abstract

Addition of thiourea (TU) or dimethylthiourea (DMTU) decreased killing of Staphylococcus aureus, 502A, and decreased concentrations of hydrogen peroxide (H2O2), and hydroxyl radical (.OH), but not superoxide anion (O2-.) or lysozyme concentrations, in mixtures containing human neutrophils in vitro. Addition of TU or DMTU also decreased concentrations of H2O2, .OH, or hypochlorous acid (HOCl) in neutrophil-free mixtures exposed to beta-D-glucose and glucose oxidase, gamma irradiation, or HOCl, respectively. Our results suggest that TU or DMTU can decrease neutrophil-mediated killing of bacteria by inhibiting O2 metabolite-dependent bactericidal mechanisms.

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Blood Bactericidal ActivityHumansHydrogen PeroxideIn Vitro TechniquesL-Lactate DehydrogenaseMuramidaseNeutrophilsStaphylococcus aureusSuperoxidesThioureaJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J H Jackson, E M Berger, J E Repine. 1988. Thiourea and dimethylthiourea decrease human neutrophil bactericidal function in vitro.. https://doi.org/10.1007/bf00919443

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