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PubMed · 2372122

A general method of optimizing automated DNA synthesis to decrease chemical consumption to less than half.

A T Yeung·C G Miller
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Abstract

An automated DNA synthesis protocol (FC3) has been optimized to decrease the cost of chemical consumption by two- to threefold. Anion-exchange chromatography at pH 12.5 in a Mono Q (Pharmacia) column was used to analyze the oligonucleotides we synthesized. From these analyses, we formulated the FC3 program and a procedure by which a DNA synthesizer designer or operator can use 18-base-long homopolymers of A, G, C, and T to optimize the reaction times, reagent concentrations, solvent wash conditions, and the many steps in the synthesis cycle.

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AutomationBase SequenceChromatography, Ion ExchangeDNAElectrophoresis, Polyacrylamide GelOligonucleotidesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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A T Yeung, C G Miller. 1990-05-15. A general method of optimizing automated DNA synthesis to decrease chemical consumption to less than half.. https://doi.org/10.1016/0003-2697(90)90418-9

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