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PubMed · 2340942

["Insect bite" and Borrelia].

P Van Durme·C Mestdagh·G Bigaignon·M Ledoux
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Abstract

A case of insect bite with a peculiar evolution is presented. The simultaneous inoculation of Borrelia burgdorferi and the treatment are discussed.

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Borrelia burgdorferi GroupFacial DermatosesFemaleHumansInsect Bites and StingsLyme DiseaseMiddle AgedSkin UlcerCase ReportsEnglish AbstractJournal Article

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P Van Durme, C Mestdagh, G Bigaignon, M Ledoux. 1990. ["Insect bite" and Borrelia].. https://pubmed.ncbi.nlm.nih.gov/2340942/

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Differentiation of Borrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis.

Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S-23S intergenic space region PCR product. A differentiation of B. garinii, B. afzelii, and B. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S-23S intergenic space region PCR product showed no difference in specificity of species identification.

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