PubMed · 1838770
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J D Spikes. 1991. What does HPD really mean?. https://doi.org/10.1016/1011-1344(91)80023-b
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The spectral properties of photosensitizer HpD, human serum albumin (HSA) and their complex have been studied. The results show that HpD can form HpD-HSA complex with HSA in physiological condition. Compared with pure HpD, the maximum absorption and the fluorescence peaks for HpD-HSA complex had 8-10 nm red-shift. When HpD-HSA complex was excited by the light of corresponding to excitation peaks of HSA (228 and 279 nm) and HpD (394 nm), it was found that the absorption of HSA and HpD both contributed to the emission of HpD-HSA complex at 622 nm. The complex of HpD-HSA was individually excited by wavelengths corresponding to HpD absorption peaks of 402, 502, 537 and 570 nm, and the excitation efficiency of HpD-HSA at pumping wavelength of 537 and 570 nm was higher than that of HpD. The results demonstrate that the red-shift caused by the interaction of HpD and the special proteins in blood should be considered in selecting the excitation and emission wavelength in photodynamic diagnosis and therapy. The results also indicate that the excitation efficiency of porphyrin-protein complex in vivo is higher than that of HpD in vitro when a longer wavelength light corresponding to the week absorption peaks of porphyrin-protein complex is used in photodynamic therapy.
The spectral properties of new photosensitizer ZnPcS2P2, PsD-007 and HMME, as well as traditional photosensitizer HpD have been studied by comparing their spectra in physiological saline and in physiological saline with 10 percent serum. Experimental results show that the maximum absorption peaks for PsD-007, HMME and HpD in the physiological saline with 10 percent serum appear at 400 nm in the soret region, while at 670 nm for ZnPcS2P2. The fluorescence excitation spectra closely resemble the absorption spectra. When excited by the light at the wavelengths of 413 and 514.5 nm, the fluorescence emission peaks for PsD-007, HMME and HpD appear at 625 and 690 nm, respectively. The fluorescent excitation efficiency of the same photosensitizer with the same concentration excited by the light at the wavelength of 413 nm is about three fold higher than that at 514.5 nm. Furthermore, the fluorescent excitation efficiency is the highest for HMME, but is lower for HpD and lowest for PsD-007. These results are significant in the selection of photosensitizers for photodynamic diagnosis and therapy.
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