Functional and quantitative proteomics using SILAC.

Matthias Mann

doi:10.1038/nrm2067

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Functional and quantitative proteomics using SILAC.

Matthias Mann

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Abstract

Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

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Amino Acids Animals Humans Isotope Labeling Mass Spectrometry Protein Interaction Mapping Proteins Proteomics Research Support, Non-U.S. Gov't Review Journal Article

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Matthias Mann. 2006. Functional and quantitative proteomics using SILAC.. https://doi.org/10.1038/nrm2067

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