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PubMed · 16970955

Ethanol beverages containing polyphenols decrease nuclear factor kappa-B activation in mononuclear cells and circulating MCP-1 concentrations in healthy volunteers during a fat-enriched diet.

Luis Miguel Blanco-Colio·Begoña Muñoz-García·Jose Luis Martín-Ventura·Luis Antonio Alvarez-Sala·Manuela Castilla·Ana Bustamante·Rosa María Lamuela-Raventós·Juan Gómez-Gerique·Arturo Fernández-Cruz·Jesús Millán·Jesús Egido
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Abstract

AIMS: Different epidemiological studies have demonstrated that some ethanol containing beverages intake could be associated with a reduction of cardiovascular mortality, effect attributed in part to its antioxidant properties. Nuclear factor-kappa B (NF-kappaB) is a redox sensitive transcription factor implicated in the pathogenesis of atherosclerosis. We have examined the effect of four different ethanol containing beverages on the activation of NF-kappaB in peripheral blood mononuclear cells (PBMC) and circulating concentrations of monocyte chemoattractant protein-1 (MCP-1) in healthy volunteers receiving a fat-enriched diet. METHODS AND RESULTS: Sixteen volunteers received 16 g/m(2) of ethanol in form of red wine, spirits (vodka, rum, and brandy) or no ethanol intake along with a fat-enriched diet during 5 days and all of them took all alcohols at different periods. NF-kappaB activation (electrophoretic mobility shift assay) and circulating MCP-1 levels (ELISA) were examined in blood samples taken before and after 5 days of ethanol intake. Subjects receiving a fat-enriched diet had increased NF-kappaB activation in PBMC at day 5. Furthermore, MCP-1 levels were increased in plasma at day 5. Red wine intake and some ethanol beverages containing polyphenols (brandy and rum) prevented NF-kappaB activation and decreased MCP-1 release. CONCLUSION: Consumption of moderate amounts of alcoholic drinks containing polyphenols decreases NF-kappaB activation in PBMCs and MCP-1 plasma levels during a fat-enriched diet. Our results provide additional evidence of the anti-inflammatory effects of some ethanol containing beverages, further supporting the idea that its moderate consumption may help to reduce overall cardiovascular mortality.

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AdultAlcoholic BeveragesAnti-Inflammatory AgentsChemokine CCL2Dietary FatsFemaleFlavonoidsHumansLeukocytes, MononuclearMaleNF-kappa BPhenolsPolyphenolsJournal ArticleResearch Support, Non-U.S. Gov't

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Luis Miguel Blanco-Colio, Begoña Muñoz-García, Jose Luis Martín-Ventura, Luis Antonio Alvarez-Sala, Manuela Castilla, Ana Bustamante, Rosa María Lamuela-Raventós, Juan Gómez-Gerique, Arturo Fernández-Cruz, Jesús Millán, Jesús Egido. 2006-09-12. Ethanol beverages containing polyphenols decrease nuclear factor kappa-B activation in mononuclear cells and circulating MCP-1 concentrations in healthy volunteers during a fat-enriched diet.. https://doi.org/10.1016/j.atherosclerosis.2006.07.035

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