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Live cell refractometry using microfluidic devices.

Niyom Lue·Gabriel Popescu·Takahiro Ikeda·Ramachandra R Dasari·Kamran Badizadegan·Michael S Feld
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Abstract

Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.

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Cell ShapeHeLa CellsHumansMicrofluidic Analytical TechniquesMicroscopy, Phase-ContrastRefractometryJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R Dasari, Kamran Badizadegan, Michael S Feld. 2006-09-15. Live cell refractometry using microfluidic devices.. https://doi.org/10.1364/ol.31.002759

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