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PubMed · 168269

An improved quantitative micro-complement fixation test.

M Cikes
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Abstract

The technical procedures for a simple quantitative micro-complement (C) fixation test are described. Major advantages of the present technique compared with the previously described method are: a) a simple measurement of the residual hemolytic activity of C by counting the radioactivity released from 51Cr-labeled sensitized sheep erythrocytes (51Cr-EA); b) an increased sensitivity of the test, brought about by the use of a relatively small number of 51CR-EA per reaction volume; and c) an increased specificity of the test, achieved by maintaining a constant amount of C available for the specific antigen--antibody reaction.

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AdenoviridaeAnimalsAntigen-Antibody ReactionsCell LineCell-Free SystemCells, CulturedChromium RadioisotopesComplement Fixation TestsCricetinaeErythrocytesGuinea PigsHemolysisImmune SeraNeoplasms, ExperimentalRabbitsSheepSimian virus 40Journal Article

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M Cikes. 1975. An improved quantitative micro-complement fixation test.. https://doi.org/10.1016/0022-1759(75)90085-x

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