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PubMed · 16806926

Fluorogenic ester substrates to assess proteolytic activity.

Laurent Mugherli·Olga N Burchak·François Chatelain·Maxim Y Balakirev
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Abstract

The synthesis of a new type of fluorogenic ester substrates is described. Prepared from fluorescein in three steps with common commercially available precursors, they all generate bright green fluorescence upon proteolysis. Their particular structure allows the same substrate be used to report enzymatic activity of various proteases from serine and cysteine superfamilies. The substrate cleavage is sensitive to specific protease inhibitors providing a tool for inhibitor screening.

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EstersFluoresceinKineticsMolecular StructurePeptide HydrolasesSubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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Laurent Mugherli, Olga N Burchak, François Chatelain, Maxim Y Balakirev. 2006-06-27. Fluorogenic ester substrates to assess proteolytic activity.. https://doi.org/10.1016/j.bmcl.2006.06.037

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