Visualization of molecular interactions by fluorescence complementation.

Tom K Kerppola

doi:10.1038/nrm1929

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Visualization of molecular interactions by fluorescence complementation.

Tom K Kerppola

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Abstract

The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.

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Microscopy, Fluorescence Protein Interaction Mapping Proteins Spectrometry, Fluorescence Ubiquitin Journal Article Review

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Tom K Kerppola. 2006. Visualization of molecular interactions by fluorescence complementation.. https://doi.org/10.1038/nrm1929

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