Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 16436602

Dendrites contain a spacing pattern.

Aaron B Taylor·Justin R Fallon
View original source ↗Export metadata ↓

Abstract

The distinctive branching patterns of dendritic arbors are essential for neuronal information processing. The final shape of an arbor is the result of intrinsic and extrinsic factors. However, the cellular mechanisms that underlie branch patterning are unknown. In many biological systems, locally acting factors are intrinsically organized into spacing patterns that guide patterned morphogenesis. Here, we show that neurons contain two types of periodic and regular elements (PADREN1s and PADREN2s) that are arranged into a spacing pattern. The wavelength of the pattern is approximately 20 microm. Dendritic branches occur preferentially within PADREN1s, and specific PADREN lengths correspond to specific arbor types. The lengths of the PADRENs also change over time and can be modified by activity. However, PADRENs are intrinsically organized, possibly by a reaction-diffusion process. PADRENs reveal a previously unrecognized level of neuronal organization that may provide insight into how the distinct branching patterns of the dendrites are intrinsically organized.

Explore related subjects

6-Cyano-7-nitroquinoxaline-2,3-dioneAction PotentialsAnimalsCells, CulturedCerebellumDendritesDiffusionExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsGlutamic AcidHippocampusMiceMicrotubule-Associated ProteinsMicrotubulesMorphogenesisNeocortexNeuronsNocodazolePaclitaxelPotassium ChloridePyramidal CellsRatsSodium Channel BlockersStaining and LabelingTetrodotoxinValineJournal ArticleResearch Support, N.I.H., Extramural

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Aaron B Taylor, Justin R Fallon. 2006-01-25. Dendrites contain a spacing pattern.. https://doi.org/10.1523/jneurosci.4424-05.2006

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Molecular mechanisms of neurite growth with AMPA receptor potentiation.

Positive allosteric modulation of AMPA receptor function has therapeutic potential in a number of psychiatric disorders and neurodegenerative diseases. AMPA receptor potentiators can induce neurite sprouting in vivo. Using a strategy of combined morphological and biochemical analyses, we investigated the effect of the AMPA receptor potentiator LY404187 on neurite growth in the SH-SY5Y human neuroblastoma cell line. LY404187 (0.1-10 microM) increased average neurite length and neurofilament expression when co-administered with s-AMPA. Co-incubation with s-AMPA and LY404187 also increased Trk receptor expression. All actions of LY404187 were sensitive to AMPA receptor blockade by the selective antagonist CNQX (10 microM). Antibody sequestration of BDNF attenuated neurite growth following AMPA receptor potentiator administration, suggesting that LY404187 increases neurite length in vitro by a BDNF mediated mechanism. AMPA receptor potentiation activates multiple intracellular neurochemical cascades and the present report identifies BDNF as one key mediator of the neurotrophic effects of AMPA receptor potentiation.

6-Cyano-7-nitroquinoxaline-2,3-dione↗
PubMed2006

Opposite effects of presynaptic 5-HT3 receptor activation on spontaneous and action potential-evoked GABA release at hippocampal synapses.

5-HT(3) (serotonin type 3) receptors are targets of antiemetics, antipsychotics, and antidepressants and are believed to play a role in cognition. Nevertheless, contrasting results have been obtained with respect to their functions in the CNS and in the control of transmitter release. We used rat hippocampal neurons in single-neuron microcultures to identify the roles of presynaptic 5-HT(3) receptors at central synapses. 5-HT (10 microm) caused a transient > 10-fold increase in the frequency of miniature inhibitory postsynaptic currents without affecting amplitudes or kinetics. This effect was abolished by tropisetron (30 nm) and when Ca(2+) channels were blocked by 100 microm Cd(2+) it was mimicked and occluded when neurons were depolarized by 20 mm, but not 10 mm, K(+). Thus, activation of presynaptic 5-HT(3) receptors increased spontaneous GABA release by causing depolarization and opening of voltage-gated Ca(2+) channels. In microculture neurons, 5-HT transiently reduced action potential-evoked inhibitory autaptic currents by > 50%; this effect was blocked by tropisetron and mimicked by 20 mm, but not 10 mm, K(+). Miniature excitatory postsynaptic currents were not altered by 5-HT. Excitatory autaptic currents were tonically reduced, an effect attenuated by 5-HT(1A) antagonists. Thus, presynaptic 5-HT(3) receptors control GABA, but not glutamate, release and mediate opposite effects on spontaneous and action potential-dependent release.

6-Cyano-7-nitroquinoxaline-2,3-dione↗
PubMed2006

Comparison between basal and apical dendritic spines in estrogen-induced rapid spinogenesis of CA1 principal neurons in the adult hippocampus.

Modulation of hippocampal synaptic plasticity by estrogen has been attracting much attention. Here, we demonstrated the rapid effect of 17beta-estradiol on the density and morphology of spines in the stratum oriens (s.o., basal side) and in the stratum lacunosum-moleculare (s.l.m., apical side) by imaging Lucifer Yellow-injected CA1 neurons in adult male rat hippocampal slices, because spines in s.o. and s.l.m. have been poorly understood as compared with spines in the stratum radiatum. The application of 1nM estradiol-induced a rapid increase in the density of spines of pyramidal neurons within 2h. This increase by estradiol was blocked by Erk MAP kinase inhibitor and estrogen receptor inhibitor in both regions. Effect of blockade by agonists of AMPA receptors and NMDA receptors was different between s.o. and s.l.m. In both regions, ERalpha agonist PPT induced the same enhancing effect of spinogenesis as that induced by estradiol.

6-Cyano-7-nitroquinoxaline-2,3-dione↗