Highly parallel microbial diagnostics using oligonucleotide microarrays.

Alexander Loy; Levente Bodrossy

doi:10.1016/j.cccn.2005.05.041

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Highly parallel microbial diagnostics using oligonucleotide microarrays.

Alexander Loy·Levente Bodrossy

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Abstract

Oligonucleotide microarrays are highly parallel hybridization platforms, allowing rapid and simultaneous identification of many different microorganisms and viruses in a single assay. In the past few years, researchers have been confronted with a dramatic increase in the number of studies reporting development and/or improvement of oligonucleotide microarrays for microbial diagnostics, but use of the technology in routine diagnostics is still constrained by a variety of factors. Careful development of microarray essentials (such as oligonucleotide probes, protocols for target preparation and hybridization, etc.) combined with extensive performance testing are thus mandatory requirements for the maturation of diagnostic microarrays from fancy technological gimmicks to robust and routinely applicable tools.

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Bacteria Environmental Microbiology Genetic Markers Genome Humans Infections Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Sensitivity and Specificity Viruses Journal Article Research Support, Non-U.S. Gov't Review

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Alexander Loy, Levente Bodrossy. 2005-08-26. Highly parallel microbial diagnostics using oligonucleotide microarrays.. https://doi.org/10.1016/j.cccn.2005.05.041

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