SARS coronavirus detection methods.

Susanna K P Lau; Xiao-Yan Che; Patrick C Y Woo; Beatrice H L Wong; Vincent C C Cheng; Gibson K S Woo; Ivan F N Hung; Rosana W S Poon; Kwok-Hung Chan; J S Malik Peiris; Kwok-Yung Yuen

doi:10.3201/eid1107.041045

PubMed · 16022791

SARS coronavirus detection methods.

Susanna K P Lau·Xiao-Yan Che·Patrick C Y Woo·Beatrice H L Wong·Vincent C C Cheng·Gibson K S Woo·Ivan F N Hung·Rosana W S Poon·Kwok-Hung Chan·J S Malik Peiris·Kwok-Yung Yuen

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Abstract

Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription-polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.

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Enzyme-Linked Immunosorbent Assay Feces Humans Nucleocapsid Proteins Reverse Transcriptase Polymerase Chain Reaction Severe acute respiratory syndrome-related coronavirus Sensitivity and Specificity Severe Acute Respiratory Syndrome Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Susanna K P Lau, Xiao-Yan Che, Patrick C Y Woo, Beatrice H L Wong, Vincent C C Cheng, Gibson K S Woo, Ivan F N Hung, Rosana W S Poon, Kwok-Hung Chan, J S Malik Peiris, Kwok-Yung Yuen. 2005. SARS coronavirus detection methods.. https://doi.org/10.3201/eid1107.041045

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