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PubMed · 1591775

c-abl has a sequence-specific enhancer binding activity.

R Dikstein·D Heffetz·Y Ben-Neriah·Y Shaul
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Abstract

The enhancers of several distinct viruses contain a common functional element, termed EP. This element binds ubiquitous cellular proteins and generates specific complexes in gel retardation analysis. Ultraviolet cross-linking and Southwestern analysis showed that a 140 kd polypeptide is the major EP DNA-binding protein. Using a combination of DNA binding and immunological techniques, we have identified the c-abl protein in a nuclear complex that binds to the EP element. abl was found to have both a specific and high affinity DNA binding activity. The ability to bind DNA is abolished in the mutant abl protein, p210bcr-abl, consistent with its cytoplasmic localization in chronic myelogenous leukemia.

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Base SequenceDNA ProbesDNA, ViralEnhancer Elements, GeneticGenes, ablHeLa CellsHepatitis B virusHumansMolecular Sequence DataPrecipitin TestsProto-Oncogene Proteins c-ablJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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R Dikstein, D Heffetz, Y Ben-Neriah, Y Shaul. 1992-05-29. c-abl has a sequence-specific enhancer binding activity.. https://doi.org/10.1016/0092-8674(92)90287-m

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