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Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age.

Mohammed S Al-Marhoon·Sheila Nunn·Roger W Soames
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Abstract

BACKGROUND AND AIMS: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity. METHODS: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction. RESULTS: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61+/-2.8 degrees ) and H pylori-negative patients (65.5+/-3.0 degrees ). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5+/-3.0 degrees , 58.6+/-3.6 degrees and 63.4+/-4.9 degrees , respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3+/-8.3 degrees , 61.1+/-6.1 degrees and 63.6+/-2.2 degrees , respectively; P=0.70) and in patients without atrophy (71.1+/-8 degrees , 59.6+/-4 degrees and 66+/-2 degrees , respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306). CONCLUSIONS: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.

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AdultAge FactorsAgedAntigens, BacterialBacterial ProteinsFemaleGastric MucosaHelicobacter InfectionsHelicobacter pyloriHumansHydrophobic and Hydrophilic InteractionsMaleMiddle AgedSeverity of Illness IndexSex FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Mohammed S Al-Marhoon, Sheila Nunn, Roger W Soames. 2005. Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age.. https://doi.org/10.1155/2005%2F842354

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