PubMed · 15674396
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
Inder M Verma. 2005. Interview with Dr Verma.. https://doi.org/10.1038/sj.gt.3302466
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Recently, magnetic nanoparticles have attracted attention because of their potential in biomedicine. They can be applied to special medical techniques, such as separation, immunoassay, magnetic resonance imaging (MRI), drug delivery, and hyperthermia, and so on. But these nanoparticles only have one or two kinds of functions and cannot realize multipurpose. Therefore, we propose that it is need to constitute magnetic multifunctional nanoplatforms, which not only have delivery property of gene vector and drug carrier, but also have heating property of thermseeds in the alternative magnetic field. Recent trials have proved that gene therapy combined with chemotherapy and hyperthermia achieves better results than single therapy strategy. But this is difficult in clinical practice. With the development of the magnetic multifunctional nanoplatforms, the combined treatment is feasible in clinical practice. This nanoplatforms is versatile, and can be applied to wide fields. The use of magnetic multifunctional nanoplatforms will further improve medical techniques.
Explore the source record for details and available documents.
For centuries mankind led a difficult battle against viruses, the smallest infectious agents at the surface of the earth. Nowadays it is possible to use viruses for our benefit, both at a prophylactic level in the production of vaccines and at a therapeutic level in the promising field of gene therapy. Retroviruses were discovered at the end of the 19th century and constitute one of the most effective entities for gene transfer and insertion into the genome of mammalian cells. This attractive feature has intensified research in retroviral vectors development and production over the past years, mainly due to the expectations raised by the concept of gene therapy. The demand for high quality retroviral vectors that meet standard requisites from the regulatory agencies (FDA and EMEA) is therefore increasing, as the technology has moved into clinical trials. The development of safer producer cell lines that can be used in large-scale production will result in the production of large quantities of retroviral stocks. Cost-efficient and scalable purification processes are essential for production of injectable-grade preparations to achieve final implementation of these vectors as therapeutics. Several preparative purification steps already established for proteins can certainly be applied to retroviral vectors, in particular membrane filtration and chromatographic methods. Nevertheless, the special properties of these complex products require technological improvement of the existing purification steps and/or development of particular purification steps to increase productivity and throughput, while maintaining biological activity of the final product. This review focuses on downstream process development in relation to the retroviral vectors characteristics and quality assessment of retroviral stocks for intended use in gene therapy.