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PubMed · 15640020

[A new blocking method for DNA chip].

Qun He·Yu-Jie Zhao
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Abstract

Suggested a new blocking method for DNA microarray: 1-iodo-propane. We compared the difference between the two block reagents and the blocking results in different blocking time. The results showed that 1-I-propane is batter-blocking reagent than succinic anhydride in DNA chip. 1h blocking time is enough for blocking.

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Hydrocarbons, IodinatedOligonucleotide Array Sequence AnalysisSuccinic AnhydridesComparative StudyEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Qun He, Yu-Jie Zhao. 2004. [A new blocking method for DNA chip].. https://pubmed.ncbi.nlm.nih.gov/15640020/

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Solubilization and methylation of HgS, PbS, and SnS by iodomethane, a model experiment for the aquatic environment.

Iodomethane is produced in the marine environment by algae and plankton. Model experiments have shown that iodomethane can dissolve a series of metal sulphides, in agreement with some of the results mentioned in the relative literature. While no dissolution of SnS was observed, PbS and HgS were dissolved proportionally with the iodomethane concentration. The two forms of HgS (cinnabar and metacinnabar) behaved differently, with metacinnabar being more soluble than cinnabar. Moreover, iodomethane can methylate lead and tin and mercury can be methylated when methyllead and methyltin compounds are present in the model system. The methylated species were identified using gas chromatography, and there is evidence that lead and tin undergo the same methylation mechanism. Preliminary experiments with natural sediments were performed, but no dissolution of Pb, Sn or Hg was observed. CAPSULE: Iodomethane can solubilize Pb and Hg sulphides, and methylate Pb and Sn, and (indirectly) Hg.

Hydrocarbons, Iodinated↗