PubMed · 15573560
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John L Hierbaum. 2004. 'Power' tools for your program.. https://pubmed.ncbi.nlm.nih.gov/15573560/
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Combined confocal scanning ophthalmoscopy/en face T-scan-based ultrahigh-resolution optical coherence tomography (OCT) of the human retina in vivo is reported for the first time to our knowledge. The system uses a superluminescent diode-based broadband source, which gives an axial resolution of 3.2 microm in the retina. We demonstrate acquisition of T-scan-based OCT B-scan and simultaneous confocal/C-scan images of the human retina of large lateral size (covering a field of up to 20 degrees ) at a frame rate of 2Hz.
A simple and cost-effective method for real-time imaging in confocal microscopy is proposed. Spectrally encoded slit confocal microscopy (SESCoM) uses a spectral encoding technique together with a confocal slit aperture to achieve two-dimensional images. Simulation and experimental results of the SESCoM's axial and lateral performances are presented. The measured FWHM of the axial response is 1.15 mum when an objective with a NA of 0.95 is used. FWHMs of the lateral line spread functions are measured to be 236 and 244 nm along the x and y directions, respectively. Both the axial and the lateral experimental results agree well with the simulation results.
A simple setup for generating evanescent Bessel beams is proposed. When a radially polarized beam is strongly focused onto a dielectric-metal interface, the entire beam is p-polarized with respect to the dielectric-metal interface, enabling excitation of surface plasmons from all directions. The angular selectivity of surface plasmon excitation mimics the function of an axicon, leading to an evanescent nondiffracting Bessel beam. The created evanescent Bessel beam may be used as a virtual probe for near-field optical imaging and sensing applications.