PubMed · 15377032
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
Mieko Shiwa. 2004. [ProteinChip system].. https://pubmed.ncbi.nlm.nih.gov/15377032/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
A simple method to purify recombinant proteins is described by fusing a target protein with an intein and an elastin-like polypeptide that only requires NaCl, dithiothreitol, and a syringe filter to isolate the target protein from Escherichia coli lysate. This tripartite fusion system enables rapid isolation of the target protein without the need for affinity chromatography for purification or proteases for cleavage of the target protein from the fusion. The elastin-like polypeptide tag imparts reversible phase transition behavior to the tripartite fusion so that the fusion protein can be selectively aggregated in cell lysate by the addition of NaCl. The aggregates are isolated by microfiltration and resolubilized by reversal of the phase transition in low ionic strength buffer. After resolubilizing the fusion protein, the intein is activated to cleave the target protein from the elastin-intein tag, and the target protein is then isolated from the elastin-intein fusion by an additional phase transition cycle.
Explore the source record for details and available documents.
High pressures, in the kilobar range, are now used in liquid chromatography. Basic equations from mechanics are applied to investigate the stress state in several idealized chromatography tubes, and these stresses are evaluated with respect to the maximum allowable stresses predicted by several methods used in pressure vessel design. An analytical solution is developed for the dimensional changes of idealized tubes subjected to internal pressure, and the analytical solutions used to verify the results from a numerical approximation. Numerical approximations are then used to explore the effects of the end restraint provided by the end frits. Conclusions are derived regarding the requirements for a safe operation of these high pressure chromatography tubes.