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PubMed · 15211493

Three-dimensional microarray platform applied to single nucleotide polymorphism analysis.

Donald I Stimpson·Sheila M Knepper·Miho Shida·Kimimichi Obata·Hideji Tajima
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Abstract

Bio-Strand, Inc., has developed a novel DNA microarray platform utilizing a three-dimensional (3D) DNA format. DNA probes or polymerase chain reaction (PCR) products are spotted onto a thread-like scaffold, which is then wound onto a cylindrical core. By wrapping the thread around the core, high efficiencies are achieved in sample analysis. Using allele-specific oligo (ASO) competitive hybridization (with Cy5 fluorescently labeled sequences), hybridized arrays are visualized using a helium-neon (HeNe) laser and quantitated/scored. The method can readily detect single nucleotide differences. We demonstrate the use of this Bio-Strand 3D array in the analysis of a single nucleotide polymorphism (SNP).

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CarbocyaninesDNADNA ProbesHumansNylonsOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionPolymorphism, Single NucleotideSensitivity and SpecificityJournal Article

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Donald I Stimpson, Sheila M Knepper, Miho Shida, Kimimichi Obata, Hideji Tajima. 2004-07-05. Three-dimensional microarray platform applied to single nucleotide polymorphism analysis.. https://doi.org/10.1002/bit.20087

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