High-throughput preparative process utilizing three complementary chromatographic purification technologies.

Manuel Ventura; William Farrell; Christine Aurigemma; Kathleen Tivel; Michael Greig; Jeffrey Wheatley; Alex Yanovsky; Kenneth Eric Milgram; David Dalesandro; Raylyn DeGuzman; Phuong Tran; Leslie Nguyen; Loanne Chung; Ole Gron; Charles A Koch

doi:10.1016/j.chroma.2004.02.071

PubMed · 15139408

High-throughput preparative process utilizing three complementary chromatographic purification technologies.

Manuel Ventura·William Farrell·Christine Aurigemma·Kathleen Tivel·Michael Greig·Jeffrey Wheatley·Alex Yanovsky·Kenneth Eric Milgram·David Dalesandro·Raylyn DeGuzman·Phuong Tran·Leslie Nguyen·Loanne Chung·Ole Gron·Charles A Koch

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Abstract

A high-throughput process was developed in which wells in plates generated from parallel synthesis are automatically channeled to an appropriate purification technique using analytical data as a guide. Samples are directed to either of three fundamentally different preparative techniques: HPLC with UV-triggered fraction collection, supercritical fluid chromatography (SFC) with UV-triggered fraction collection, or HPLC with MS-triggered fraction collection. Automated analysis of the analytical data identifies the product compound mass and creates work lists based on chromatographic properties exhibited in the data so that each preparative instrument cherry picks the appropriate list of samples to purify when a preparative-scale plate is loaded.

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Chromatography, High Pressure Liquid Chromatography, Supercritical Fluid Spectrophotometry, Ultraviolet Journal Article

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Manuel Ventura, William Farrell, Christine Aurigemma, Kathleen Tivel, Michael Greig, Jeffrey Wheatley, Alex Yanovsky, Kenneth Eric Milgram, David Dalesandro, Raylyn DeGuzman, Phuong Tran, Leslie Nguyen, Loanne Chung, Ole Gron, Charles A Koch. 2004-05-14. High-throughput preparative process utilizing three complementary chromatographic purification technologies.. https://doi.org/10.1016/j.chroma.2004.02.071

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