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PubMed · 15097996

Encoded self-assembling chemical libraries.

Samu Melkko·Jörg Scheuermann·Christoph E Dumelin·Dario Neri
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Abstract

The isolation of molecules capable of high-affinity and specific binding to biological targets is a central problem in chemistry, biology and pharmaceutical sciences. Here we describe the use of encoded self-assembling chemical (ESAC) libraries for the facile identification of molecules that bind macromolecular targets. ESAC technology uses libraries of organic molecules linked to individual oligonucleotides that mediate the self-assembly of the library and provide a code associated with each organic molecule. After panning ESAC libraries on the biomolecular target of interest, the 'binding code' of the selected compounds can be 'decoded' by a number of experimental techniques (e.g., hybridization on oligonucleotide microarrays). The potential of this technology was demonstrated by the affinity maturation (>40-fold) of binding molecules to human serum albumin and bovine carbonic anhydrase, leading to binders with dissociation constants in the nanomolar range.

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AlbuminsAnimalsBase SequenceCarbonic AnhydrasesCattleDNA PrimersHumansOligonucleotidesProtein BindingJournal ArticleResearch Support, Non-U.S. Gov't

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Samu Melkko, Jörg Scheuermann, Christoph E Dumelin, Dario Neri. 2004-04-18. Encoded self-assembling chemical libraries.. https://doi.org/10.1038/nbt961

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