Aminoacylase pellets.

K I Hirano; I Karube; S Suzuki

doi:10.1002/bit.260190303

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Aminoacylase pellets.

K I Hirano·I Karube·S Suzuki

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Abstract

Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The aminoacylase pellets were stable in pH 4-8 but they were unstable in alkaline conditions. The aminoacylase pellets were more stable against heavy metal ions and inhibitors than native aminoacylase. However, the degree of the activation of aminoacylase with cobalt ion decreased with the immobilization. It was suggested that most of aminoacylase was covalently coupled to the mycelium with glutaraldehyde.

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Amidohydrolases Aspergillus Enzyme Activation Enzymes, Immobilized Hydrogen-Ion Concentration Temperature Journal Article

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K I Hirano, I Karube, S Suzuki. 1977. Aminoacylase pellets.. https://doi.org/10.1002/bit.260190303

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