PubMed · 14579746
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Abigail C Ting, Shern-Fwu Lee, Kai Wang. 2003. An easy setup for double slide microarray hybridization.. https://doi.org/10.2144/03354mt01
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A simple method has been developed and validated for direct, sensitive detection and specific identification of 16S rRNA. We first report our direct investigation of discrimination efficiency for sequence variations in RNA using oligonucleotide microarrays assisted by base stacking hybridization, and demonstrate that the sequence variations of double base substitution, single base substitution and single base deletion in RNA could be directly identified. With the help of tyramide signal amplification (TSA), the detection sensitivity of this method for four clinically important bacterial species was below 0.5, 5, 1 and 1 ng of total RNA, which are 100-1000 fold more sensitive than the published methods.
Nanoscale zinc oxide (ZnO) rods of diameters close to the Bohr-exciton radius ( approximately 2 nm) can be prepared from a simple acetate precursor, resulting in ligand-capped rods of ZnO, highly dispersible in nonpolar solvents. Zinc oxide, ZnO, is a wide band-gap semiconductor with applications in blue/ultraviolet (UV) optoelectronic devices and piezoelectric devices. We observe self-assembly into uniform stacks of nanorods aligned parallel to each other with respect to the long axis, and photoluminescence measurements provide evidence for one-dimensional quantum confinement.
Southern analysis with rpl5 and rps14 mtDNA gene probes of Solanum tuberosum, S. commersonii and a sample of somatic hybrids detected polymorphisms between parents and the appearance of a novel restriction fragment in various hybrids. In one of them, detailed mtDNA analyses revealed various configurations of the rpl5- rps14 region present at different stoichiometries. Multiple inter-parental recombination events across homologous sequences were assumed to have caused these rearrangements. Sequence similarity searches detected one sequence putatively involved in the recombination upstream of the rpl5 gene. The presence of a second recombinogenic sequence was inferred. We propose two models to explain the mechanism responsible for obtaining the different rpl5- rps14 arrangements shown after somatic hybridization. Variability in the rpl5- rps14 region observed in both the parental species and their somatic hybrids suggests this region is a hot spot for mtDNA rearrangements in Solanum spp.