PubMed · 13981192
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N P THOMPSON, J A PATTERSON. Solid salt bridge contact electrodes.. https://pubmed.ncbi.nlm.nih.gov/13981192/
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Scanning probe microscopy was used to monitor the resulting surface of the oriented incorporation of cytochrome c oxidase into electrode supported lipid bilayer at four crucial stages with molecular resolution. We were able to reveal the formation of a densely packed monolayer of the active ester dithio(succiniimidylepropionate) (DTSP) and the covalent linkage of the nitrilotriacetic acid (NTA) to the thiol anchored DTSP by scanning tunneling microscopy. Atomic force microscopy investigations showed that the detergent solubilized oxidase is immobilized as monomers and small aggregates via histidine residues. Finally, the reconstitution of the proteins within the supported membrane was verified. The amount of oxidase immobilized within the solid supported membrane was estimated.
We have sought to enhance the sensitivity of conductometric gas microsensors through the design and fabrication of porous, three-dimensional tin oxide nanoparticle structures. Electrostatically controlled layer-by-layer processing in aqueous solutions was used to decorate sacrificial latex microspheres with Sb:SnO2 nanoparticles. To evaluate their sensing performance, these structures were then deposited as films, via micropipetting, on MEMS micro-hot-plate platforms with interdigitated electrodes. Prior to gas testing, rapid heating of the micro-hot-plates was used to remove the sacrificial latex templates, thereby revealing a 3-D structure composed of interconnected spherical tin oxide nanoparticle shells with porous ultrathin walls. Changes in film conductance, caused by exposure to test gases (methanol, carbon monoxide, benzene, water) in a dry air background, were measured at different temperatures. Hollow nanoparticle microsphere films exhibited partial selectivity for these different gases, good dynamic range at different temperatures and gas concentrations, and good repeatability and stability over long runs. These films also yielded approximately 3-fold and 5-fold increases in sensitivity to methanol when compared to SnO2 polycrystalline chemical vapor deposition films and Sb:SnO2 microporous nanoparticle films, respectively. Gains in sensitivity are attributed to the multiscale porous architecture of the hollow microsphere films. This architecture promotes gas diffusion and increases the active surface area.