Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 13970691

Electrophoretic method for characterizing thyroid function.

S BERGER·M S GOLDSTEIN·B E METZGER
View original source ↗Export metadata ↓

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Blood Protein ElectrophoresisHyperthyroidismHypothyroidismIodine IsotopesThyroid Function TestsThyroxineBLOOD PROTEIN ELECTROPHORESISHYPERTHYROIDISMHYPOTHYROIDISMIODINE ISOTOPES, DIAGNOSTICTHYROID FUNCTION TESTSTHYROXINJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

S BERGER, M S GOLDSTEIN, B E METZGER. 1962-10-18. Electrophoretic method for characterizing thyroid function.. https://doi.org/10.1056/nejm196210182671603

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2005

Ocular copper deposition associated with monoclonal gammopathy of undetermined significance: case report.

To report a case of ocular copper deposition in both eyes at the level of Descemet's membrane associated with a monoclonal gammopathy of undetermined significance (MGUS). A 49-year-old white woman had golden-brown metallic dust-like deposits on Descemet's membrane of both eyes. A systemic examination revealed an elevated serum copper, normal serum ceruloplasmin and a normal level of total protein. Serum protein electrophoresis demonstrated a single peak (M-spike) in the gamma region (M-protein in serum = 11 g/l). Flow cytometric analysis of the marrow aspirate identified a monoclonal plasma cell population that represents approximately 2% of the total marrow cells consistent with the diagnosis of monoclonal gammopathy of undetermined significance. Copper deposits at the level of Descemet's membrane may be a finding in a patient with monoclonal gammopathy of undetermined significance.

Blood Protein Electrophoresis↗
PubMed2005

Capillary electrophoresis for monitoring the effects of plasmapheresis: a feasibility study.

Plasmapheresis is an established method to treat patients with large monoclonal gammopathies (M-components) that increases the blood viscosity. During plasmapheresis the patient plasma is substituted with a mixture of saline, albumin and normal plasma. The treatment results in a reduction of the M-component in the circulation. Plasmapheresis causes changes in several proteins, which makes monitoring by nephelometric or turbidimetric analysis of specific proteins less suitable. Quantification of paraproteins by nephelometric assays are also known to be associated with technical problems. An alternative could be agarose gel electrophoresis but this method is too slow to permit monitoring during the plasmapheresis. Capillary electrophoresis of plasma proteins can be performed in less than 10 min. We have used the CAPILLARYS capillary electrophoresis system (Sebia, Paris, France) to monitor the effect of plasmapheresis treatment on 22 occasions. The mean reduction in M-component size was 51%, range 33-61%. Capillary electrophoresis is a rapid and inexpensive method that could be used to monitor the effect of plasmapheresis during the actual treatment.

Blood Protein Electrophoresis↗
PubMed2005

Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys.

We studied the linearity and detection limits of the capillary zone electrophoresis system Capillarys in the measurement of serum monoclonal protein. Three monoclonal proteins with different isotypes and electrophoretic migrations were diluted with a hypo-gamma-globulinemic polyclonal serum pool. Mathematical linearity was observed for all monoclonal protein isotypes in the ranges studied without removing the polyclonal gamma-globulin background. Theoretical concentrations of 0.43, 0.89 and 0.33 g/L for monoclonal proteins immunoglobulin (Ig)G, IgA and IgM, respectively, gave a discernible spike by Capillarys, although they were measured as 0.76, 1.09 and 0.76g/L, respectively. We observed overestimation of monoclonal protein inversely correlated to concentrations below 15 g/L. All these limitations have to be taken into account when monitoring monoclonal proteins, because the loss of linearity and the protein background may hide an increase in concentration at low levels.

Blood Protein Electrophoresis↗