Lambda CM8, a human sequence with putative centromeric function, does not map to the centromere but is present in one to two copies at 9qter.

N I McGill; J Fantes; H Cooke

doi:10.1093/hmg/1.9.749

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Lambda CM8, a human sequence with putative centromeric function, does not map to the centromere but is present in one to two copies at 9qter.

N I McGill·J Fantes·H Cooke

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Abstract

A DNA fragment isolated from a human genomic library, was reported to be present at all human centromeres and present at 16-32 copies per genome. Reintroduction of this DNA into mammalian cells as a concatenated phage clone gave rise to dicentric chromosomes which gave rise to a new, stable, chromosome. Taken together these observations could mean that this DNA is part of a native centromere. We have reexamined the location and copy number of this sequence and find it to be present at 1-2 copies per genome with a single site of in situ hybridisation at 9qter.

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Blotting, Southern Centromere Chromosome Mapping Chromosomes, Human, Pair 9 Cloning, Molecular DNA Deoxyribonuclease EcoRI Genomic Library HeLa Cells Humans In Situ Hybridization Lymphocytes Restriction Mapping Journal Article Research Support, Non-U.S. Gov't

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N I McGill, J Fantes, H Cooke. 1992. Lambda CM8, a human sequence with putative centromeric function, does not map to the centromere but is present in one to two copies at 9qter.. https://doi.org/10.1093/hmg%2F1.9.749

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