PubMed · 13012321
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W NEUWEILER. 1952-09-01. [Mastitis and aureomycin].. https://pubmed.ncbi.nlm.nih.gov/13012321/
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A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C18 extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 microg l(-1) OTC, TC, and CTC were 0.21, 0.20, and 0.28 microg l(-1). Method detection limits in lagoon water samples fortified at 20 microg l(-1) for OTC, TC, and CTC were 3.6, 3.1, and 3.8 microg l(-1). Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.
We have developed a genetic system allowing for precise regulation of plasmid copy number in Escherichia coli cells. A cloning vector based on this system is described in this article. The pTC lambda 3 plasmid is a lambda replicon, but transcription controlling initiation of plasmid DNA replication starts from the PtetA promoter instead of phage lambda PR promoter. Additionally, activity of PtetA promoter is negatively controlled by the TetR repressor whose gene is located on the same plasmid vector and is induced by an analog of tetracycline, autoclaved chlortetracycline (aCT). Using different concentrations of the inducer it is possible to strictly regulate the copy number of pTC lambda 3 and thus the copy number of a cloned gene. The usefulness of the system for the regulatable production of a protein encoded by a gene inserted into pTC lambda 3 plasmid is demonstrated by dependence of beta-galactosidase activity on the lacZ gene dosage.
This paper describes tetracycline (TCH), oxytetracycline (OTCH) and chlortetracycline (CTCH) determination by flow injection potentiometry. In the flow system proposed TC samples are inserted in a carrier solution and converged with a Cu(II) solution of known concentration; the Cu(II) decrease due to its complexation with tetracyclines (TC) was monitored. The detector used was a homogeneous crystalline CuS/Ag2S double membrane tubular electrode with increased sensitivity. The present system allows tetracyclines determinations within a 48.1-4.8 x 10(3) ppm for TCH, 49.1-4.9 x 10(3) ppm for OTCH and 51.5-5.1 x 10(3) ppm for CTCH and a precision better than 0.4% for the three TC species. This procedure accomplishes 150-200 samples h(-1) with a Cu(II) consumption of about 13 microg determination(-1).