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PubMed · 1298031

A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

X de Lamballerie·C Zandotti·C Vignoli·C Bollet·P de Micco
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Abstract

"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.

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Chelating AgentsDNA, BacterialGene AmplificationGram-Negative BacteriaGram-Positive BacteriaIn Vitro TechniquesPolymerase Chain ReactionResins, SyntheticStreptococcus agalactiaeJournal Article

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X de Lamballerie, C Zandotti, C Vignoli, C Bollet, P de Micco. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.. https://doi.org/10.1016/0923-2508(92)90107-y

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