Spin-state selection in solid-state NMR.

Luminita Duma; Sabine Hediger; Anne Lesage; Lyndon Emsley

doi:10.1016/s1090-7807(03)00187-3

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Spin-state selection in solid-state NMR.

Luminita Duma·Sabine Hediger·Anne Lesage·Lyndon Emsley

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Abstract

Spin-state selection in solid-state NMR is demonstrated, using similar pulse sequences as used in liquid-state NMR. The different transitions of all three carbon resonances in fully 13C-labeled L-alanine are separated in different spectra. By selecting spin-states, the contribution of the J-coupling to the linewidth is removed, leading to a considerable enhancement in resolution. The spin-state-selective technique is demonstrated for magic-angle spinning frequencies from 6 to 35kHz. Other experimental conditions affecting the sensitivity of the experiments are discussed. Sensitivity losses due to the introduction of the spin-state-selective filter are shown to be acceptable. Finally, spin-state selection was used to experimentally confirm the differential broadening expected for the two transitions of the CH3 resonance.

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Alanine Carbon Isotopes Magnetics Nuclear Magnetic Resonance, Biomolecular Reproducibility of Results Sensitivity and Specificity Spin Labels Evaluation Study Journal Article Validation Study

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Luminita Duma, Sabine Hediger, Anne Lesage, Lyndon Emsley. 2003. Spin-state selection in solid-state NMR.. https://doi.org/10.1016/s1090-7807(03)00187-3

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