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PubMed · 12903342

Triplex formation using ODN conjugates with polycation comb-type copolymer.

M Ueda·M Saito·T Ishihara·T Akaike·A Maruyama
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Abstract

Polycation comb-type copolymer that is composed of polylysine backbone and dextran side chains (PLL-g-Dex) has previously been shown to stabilize duplex and triplex DNAs quite effectively. In this study, we have conjugated PLL-g-Dex with oligonucleotides (ODN) aiming to increase the triplex stabilizing efficiency of the copolymer. Here we have demonstrated that the copolymer-TFO conjugates selectively stabilize triplex DNA. Also its potential to form triplex DNA was found to be greater than PLL-g-Dex/ODN mixture.

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Base SequenceDeoxyribonuclease BamHIDeoxyribonucleases, Type II Site-SpecificDextransDrug StabilityIn Vitro TechniquesNucleic Acid ConformationNucleic Acid DenaturationOligodeoxyribonucleotidesPolyaminesPolyelectrolytesPolylysineJournal Article

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M Ueda, M Saito, T Ishihara, T Akaike, A Maruyama. 2000. Triplex formation using ODN conjugates with polycation comb-type copolymer.. https://doi.org/10.1093/nass%2F44.1.209

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