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PubMed · 12813896

Microarray-based method for combinatorial library sequence mapping and characterization.

V Abécassis·L Jaffrelo·D Rickman·L Aggerbeck·C Herbert·G Truan·D Pompon
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Abstract

Here we describe a DNA-chip-based method for high-throughput sequence mapping. This involves competitive hybridization between short and differentially labeled fluorescent oligonucleotide probes and glass-supported PCR products. Competition between an excess of oligonucleotide probes targeting the same sequence segment improves sequence discrimination and reduces sensitivity to experimental conditions such as probe concentrations, hybridization, and washing temperatures and durations. The method was found to be particularly adapted to sequence mapping of combinatorial libraries obtained by DNA shuffling between members of a gene family. We present an application of this technique for the characterization of recombination biases in combinatorial libraries used in directed evolution.

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Base SequenceBiotechnologyChromosome MappingCytochrome P-450 CYP1A1Cytochrome P-450 CYP1A2DNA, BacterialDirected Molecular EvolutionEscherichia coliGene LibraryGenes, BacterialNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisOligonucleotide ProbesPlasmidsSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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V Abécassis, L Jaffrelo, D Rickman, L Aggerbeck, C Herbert, G Truan, D Pompon. 2003. Microarray-based method for combinatorial library sequence mapping and characterization.. https://doi.org/10.2144/03346mt03

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