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PubMed · 12725792

Mapping T cell epitopes by flow cytometry.

Bodo Hoffmeister·Felix Kiecker·Lydia Tesfa·Hans-Dieter Volk·Louis J Picker·Florian Kern
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Abstract

Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays.

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Epitope MappingEpitopes, T-LymphocyteFlow CytometryHumansJournal ArticleReview

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Bodo Hoffmeister, Felix Kiecker, Lydia Tesfa, Hans-Dieter Volk, Louis J Picker, Florian Kern. 2003. Mapping T cell epitopes by flow cytometry.. https://doi.org/10.1016/s1046-2023(02)00349-3

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