Light microscopy techniques for live cell imaging.

David J Stephens; Victoria J Allan

doi:10.1126/science.1082160

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PubMed · 12677057

Light microscopy techniques for live cell imaging.

David J Stephens·Victoria J Allan

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Abstract

Since the earliest examination of cellular structures, biologists have been fascinated by observing cells using light microscopy. The advent of fluorescent labeling technologies plus the plethora of sophisticated light microscope techniques now available make studying dynamic processes in living cells almost commonplace. For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to give a brief overview of the main approaches to live cell imaging, with some mention of their pros and cons.

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Cell Physiological Phenomena Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Dyes Imaging, Three-Dimensional Light Microscopy Microscopy, Confocal Microscopy, Fluorescence Optics and Photonics Spectrometry, Fluorescence Journal Article Research Support, Non-U.S. Gov't Review

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David J Stephens, Victoria J Allan. 2003-04-04. Light microscopy techniques for live cell imaging.. https://doi.org/10.1126/science.1082160

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