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PubMed · 12480350

Microbial cell-surface display.

Sang Yup Lee·Jong Hyun Choi·Zhaohui Xu
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Abstract

Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.

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AdsorptionAmino Acid MotifsAnimalsBacterial Outer Membrane ProteinsCell MembraneCytosolGenetic TechniquesGenetic VectorsGram-Negative BacteriaProtein Structure, TertiaryRecombinant Fusion ProteinsSaccharomyces cerevisiaeJournal ArticleResearch Support, Non-U.S. Gov'tReview

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Sang Yup Lee, Jong Hyun Choi, Zhaohui Xu. 2003. Microbial cell-surface display.. https://doi.org/10.1016/s0167-7799(02)00006-9

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