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REDOR-based heteronuclear dipolar correlation experiments in multi-spin systems: rotor-encoding, directing, and multiple distance and angle determination.

Abstract

We review a variety of recently developed 1H-X heteronuclear recoupling techniques, which rely only on the homonuclear decoupling efficiency of very-fast magic-angle spinning. All these techniques, which are based on the simple rotational-echo, double-resonance (REDOR) approach for heteronuclear recoupling, are presented in a common context. Advantages and possibilities with respect to the complementary application of conventionally X and 1H-inversely detected variants are discussed in relation to the separability and analysis of multiple couplings. We present an improved and more sensitive approach to the determination of 1H-X dipolar couplings by spinning-sideband analysis, termed REREDOR, which is applicable to XHn groups in rigid and mobile systems and bears some similarity to more elaborate separated local-field methods. The estimation of medium-range 1H-X distances by analyzing signal intensities in two-dimensional REDOR correlation spectra in a model-free way is also discussed. More specifically, we demonstrate the possibility of combined distance and angle determination in H-X-H or X-H-X three-spin systems by asymmetric recoupling schemes and spinning-sideband analysis. Finally, an 1H-X correlation experiment is introduced which accomplishes high sensitivity by inverse (1H) detection and is therefore applicable to samples with 15N in natural abundance.

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BibTeXRIS

Kay Saalwächter, Ingo Schnell. REDOR-based heteronuclear dipolar correlation experiments in multi-spin systems: rotor-encoding, directing, and multiple distance and angle determination.. https://doi.org/10.1006/snmr.2002.0079

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