PubMed · 12230685
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Rajiv Shah, Richard Wormald. 2002. Glaucoma.. https://pubmed.ncbi.nlm.nih.gov/12230685/
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A method is proposed for comparing two normal means when the data consist of some observations that are correlated and others that are uncorrelated. Typically, such data will consist of one subsample in which the observations for treatment 1 and treatment 2 are independent of each other, and another subsample which consists of paired observations taken under both treatments. The proposed method is developed using asymptotic results and is evaluated using simulation. The simulation results indicate that the proposed method can provide substantial improvement in type I error rate and power when compared with standard methods of analysis.
A new simple and rapid high-performance liquid chromatographic (HPLC) method with UV detection for the determination of indapamide in biological fluids has been developed. Indapamide and internal standard were isolated from serum and whole blood samples by solid-phase extraction with RP select B cartridges. The chromatographic separation was accomplished on a reversed-phase C(8) column with a mobile phase composed of 0.1% (v/v) triethylamine in water (pH 3.5) and acetonitrile (63:37, v/v). UV detection was set at 240 nm. The calibration curves were linear in the concentration range of 10.0-100.0 ng/ml for serum, and 50.0-500.0 ng/ml for whole blood, and the limits of quantification were 10.0 and 50.0 ng/ml, respectively.
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