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PubMed · 12217606

Decrease in CD3-negative-CD8dim(+) and Vdelta2/Vgamma9 TcR+ peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection.

Gabriella Pár·Daniel Rukavina·Eckhard R Podack·Margit Horányi·Júlia Szekeres-Barthó·Géza Hegedüs·Mária Paál·László Szereday·Gyula Mózsik·Alajos Pár
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Abstract

BACKGROUND/AIMS: As chronic hepatitis C virus (HCV) infection is associated with impaired natural killer (NK) cell cytotoxicity, we examined the phenotypes and perforin expression of peripheral blood lymphocytes, as well as the effect of interferon-alpha2b (IFN-alpha2b) therapy. METHODS: Thirty-three patients had chronic hepatitis C, and of them 12 had been on IFN-alpha2b treatment. Eleven individuals had been treated earlier with IFN-alpha2b and completely cured, and eight were HCV carriers with persistently normal serum alanine aminotransferase. Three-colour flow cytometry was used to measure the percentage of CD3(+/-)CD8+, CD3+CD4+, gammadeltaTcR+, Vdelta2 TcR+, Vgamma9 TcR+, Vdelta1 TcR+, CD3-CD16+, CD3-CD56+, CD19+ and perforin-positive cells. NK cell activity was assessed by single cell cytotoxic and flow cytometric assay. RESULTS: Patients with chronic hepatitis C showed an impaired NK cytotoxicity, decreased percentage of CD3-negative-CD8dim-positive (NK subtype) and Vgamma9/Vdelta2 TcR+ as well as perforin-positive T lymphocytes, compared to controls and to those who were cured from HCV infection. IFN-alpha2b increased NK cell cytotoxicity and the percentage of perforin-positive lymphocytes. CONCLUSIONS: Our findings suggest that in chronic HCV infection a decreased percentage of CD3(-)CD8+, Vgamma9/Vdelta2 TcR+ and perforin-positive T cells and simultaneous decreased peripheral NK activity may contribute to the impaired cellular immune response and the chronicity of the disease.

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AdultAgedAntiviral AgentsCD3 ComplexCD8 AntigensFemaleFlow CytometryGene Rearrangement, delta-Chain T-Cell Antigen ReceptorGene Rearrangement, gamma-Chain T-Cell Antigen ReceptorHepatitis C, ChronicHumansImmunophenotypingInterferon alpha-2Interferon-alphaKiller Cells, NaturalLymphocyte CountMaleMembrane GlycoproteinsMiddle AgedPerforinPore Forming Cytotoxic ProteinsReceptors, Antigen, T-Cell, gamma-deltaRecombinant ProteinsClinical TrialJournal ArticleResearch Support, Non-U.S. Gov't

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Gabriella Pár, Daniel Rukavina, Eckhard R Podack, Margit Horányi, Júlia Szekeres-Barthó, Géza Hegedüs, Mária Paál, László Szereday, Gyula Mózsik, Alajos Pár. 2002. Decrease in CD3-negative-CD8dim(+) and Vdelta2/Vgamma9 TcR+ peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection.. https://doi.org/10.1016/s0168-8278(02)00218-0

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